What Holds Chromosomes Together – Max Planck Researchers Elucidate the Structure of DNA-Packaging Proteins

SMC-Kleisin-Complex. (Credit: Image courtesy of Max Planck Institute of Biochemistry)

In each cell about two meters of DNA must fit into a cell nucleus that has a diameter of only a few thousandths of a millimeter. There the DNA is organized in individual chromosomes in the form of very long filaments. If they are not equally and accurately distributed to the daughter cells during cell division, this can result in cancer or genetic defects such as trisomy 21. Therefore, to ensure safe transport of DNA during cell division the long and coiled DNA fibers must be tightly packed.

Scientists have only a sketchy understanding of this step. The SMC-kleisin protein complexes play a key role in this process. They consist of two arms (SMC) and a bridge (kleisin). The arms wrap around the DNA like a ring and thus can connect duplicated chromosomes or two distant parts of the same chromosome with each other.

Learning from bacteria
Simple organisms like bacteria also use this method of DNA packaging. The scientists, in collaboration with colleagues from South Korea, have now elucidated the structure of a precursor of human SMC-kleisin complexes of the bacterium Bacillus subtilis. The researchers showed that the bacterial SMC-kleisin complex has two arms made of identical SMC proteins that form a ring. The arms differ in their function only through the different ends of the kleisin protein with which they are connected.

In humans the DNA packaging machinery is similarly organized. “We suspect that this asymmetric structure plays an important role in the opening and closing of the ring around the DNA,” explains Frank Bürmann, PhD student in the research group ‘Chromosome Organization and Dynamics’ of Stephan Gruber. In addition, the scientists discovered how the ends of the kleisin can distinguish between correct and wrong binding sites on one pair of arms.

The cohesion of chromosomes is of critical importance for reproduction as well. In human eggs this cohesion must be maintained for decades to ensure error-free meiosis of the egg cell. Failure of cohesion is a likely cause for decreased fertility due to age or the occurrence of genetic defects such as trisomy 21. “The elucidation of the structure of SMC-kleisin protein complexes is an important milestone in understanding the intricate organization of chromosomes,” says group leader Stephan Gruber
source : http://www.biochem.mpg.de/en/news/pressroom/083_Gruber_Kleisin.html 

After B.Tech
 
 The study of Biotechnology is a blend of subjects related Biology and subjects like Mathematics, Physics, Chemistry and Engineering. A simple definition of Biotechnology can be using the properties of living organisms to produce desired products. It is a research-oriented field. So, most of the work is carried out at research institutes and laboratories.
Biotechnology finds application to a number of fields. Some of them are:
· Medical and Health Care
· Agriculture and Animal Husbandry
· Industrial Research and Development
· Food Processing
· Bio-Processing
· Chemical Engineering
· Drug Designing and Manufacture
· Environment Conservation
After completing B.Tech in Biotechnology, you can either take up a job or study further.
Job opportunities
There are many good job opportunities available after completing B.Tech Biotechnology. After multimedia industry, Biotechnology is ranked second as the growing sector with a tremendous employment potential.
After completing your B.Tech, you can seek employment in leading Indian or multi-national companies. You can apply for jobs in a range of industries dealing with pharmaceutical, chemical, bioprocessing and agriculture products. You are also eligible for opportunities in government research and development organisations.
Some companies that hire biotechnologists are Hindustan Lever, Thapar Group, Indo American Hybrid Seeds, Bincon India Limited, Bivcol, IDPL, Indian Vaccines Corporation, Hindustan Antibiotics, Sun Pharma, Cadila etc.
Some job roles you can assume are Research Scientist, Research Associate, Marketing personnel, Business Development Officer, Sales Representative, Biotech Engineer, and Lab Technician.
On the whole, it is a good career option for bright young people like you.
Higher education
A post-graduate degree will improve your job prospects further.
It will help you gain in-depth knowledge of the subject and provide the confidence required to face the job market. After completing B.Tech in Biotechnology, you can pursue M.Tech (Biotechnology), M.Tech (Bio-Informatics) or MBA (Biotechnology).
M.Tech (Biotechnology) involves study of Biotechnology in great detail.
M.Tech (Bio-Informatics) is the study of application of computer technology to the management of biological information.
Bioinformatics plays a vital role especially in drug discovery and development; computers are used to gather, store, analyse and integrate biological and genetic information
MBA (Biotechnology) provides broad introduction to Biotechnology and focuses on imparting management skills, required in chemical, pharmaceutical or allied industries.
Some of the universities offering these courses are listed below:
SRM University, Chennai offers M.Tech (Bioinformatics) and M.Tech (Biotechnology), both are two-year courses. The eligibility criterion is B.E/ B.Tech in Biotechnology/ Food and Process Engineering. Admission is based on the performance in entrance test conducted by the university.
For further details, please log on to http://www.srmuniv.ac.in
Vellore Institute of Technology University offers M.Tech (Biotechnology), M.Tech (Bio-Informatics). The eligibility criteria for these courses are B.E./B.Tech in Chemical/ Leather Technology/ Biotechnology/ Industrial Biotechnology/ Bio-chemical Engineering/ Bio-Medical Engineering/ Bio-Informatics. Admission for the Post Graduate Programmes is based on the performance in VIT Master’s Entrance Examination (VITMEE) conducted by VIT. For further details, please log on to http://www.vit.ac.in/
Amity Institute of Biotechnology offers MBA Biotechnology Management. The eligibility criterion is graduation in Biological Sciences/ Computer Sciences or equivalent qualification with minimum 55 per cent marks.
The admission is based on the performance in the entrance test (Amity Common Admission Test or CAT or MAT or GMAT) followed by group discussion and personal interview. For further details, please log on to http://www.amity.edu/
The Department of Management Science of the University of Pune also offers MBA Biotechnology Management.
Admission for this course is based on the scores attained in AIMS Test conducted by Association of Indian Management Schools.
 
 
Courses available in post graduate level are:
 
• M.Sc Biotechnology
• M.Sc( Agriculture) Biotechnology
• M.Vsc( Animal) Biotechnology
• M.Tech. Biotechnology, M.Sc./M.Vsc Veterinary Technology
• M.Sc( Marine) Biotechnology
• Medical Biotechnology
• M.Tech in Biomedical Engineering/Biotechnology
• Pharmacology
 
Depending upon the aptitude and necessity, more advanced courses such Ph.D. and Post-Doctoral Research in Biotechnology can also be pursued.

Commitment as a student and job commitment

 Biotechnology is essentially related to scientific research. Hence a good academic background in science holds a high degree of importance. With numerous students opting for this stream the level of competition has definitely risen up making it essential to put in hard work, perseverance, imagination, creativity and originality along with quality studying and working hours.

You should have thorough knowledge of all subjects and consistent every-day studying would help you achieve this. More than anything else, your basic knowledge of computer, effective oral and written communication and math problem solving ability would help you stand in good stead for future success in this stream.

Range of specializations

 Biotechnology offers an extensive range of disciplines that you can take up for specialization according to your interest and ability. Given below is a comprehensive look at the various options served to you by this stream:


• Molecular Biology
• Microbiology
• Biochemistry
• Biophysics
• Botany
• Zoology
• Pharmacology
• Physiology
• Horticulture
• Tissue Culture
• Agricultural Engineering
• Animal Sciences
• Diary Technology
• Fisheries
• Medical, Life Sciences
• Genetics
Scope of biotechnology-BIOSCOPE

 

Introduction

The applications of this branch of science are vast and simply mind-boggling. On one hand, it caters to the industrial sector such as food and beverages industry, textiles industry, biological products, medicines and pharmaceuticals while on the other hand this branch of science caters to the requirements of agriculture, animal husbandry, nutrition and environmental conservation. The list is a long and an envious one.

What’s more, the branch of science we are talking about is in itself not confined or bound as a single discipline. Rather it is an interdisciplinary branch of science that is rapidly gaining significance and opportunities for youngsters who want to explore the new frontiers of science are immense. The name of the discipline is Biotechnology.

Although, the name may suggest that this branch of science is steeped in biology but this is not the case. Apart from biology, this branch of science also assimilates diverse subjects like physics, chemistry and mathematics. Furthermore, engineering applications are also an integral constituent of biotechnology.

The concepts derived from this dissimilar mix of sciences are applied to biological matter, generally living cells, for developing new and improved biological and industrial products. Most of the work done by professionals engaged in biotechnology is concerned with research and development work in various laboratories.

Students are showing tremendous interest in biotechnology. The primary reason behind this interest emerges from the fact that the technical and procedural application of biotechnology touches a vast array of disciplines. This in turn opens a lot many job portals for students who are seeking a stable career in their life. Biotechnology, along with its many sub-fields, finds use in so many applications that many new fields have and are being derived from within it.

Step-by-Step

In opting for a specialised profession such as biotechnology, you must plan your career right from your school days. In this context, the combination of subjects of study at the 10+2 level must primarily include Biology, Chemistry, Physics and even Mathematics. Once you have finished schooling you can opt for a suitable undergraduate program (BSc, BE, BTech) in Biotechnology from various academic institutions spread across the country. The duration of a BSc program in biotechnology is three years whereas it is four years for BE and BTech courses.

The eligibility criterion for getting into an undergraduate program varies from one institution to another. In some colleges the eligibility criteria includes a 10+2 with 50% marks in Physics, Chemistry, Biology and Mathematics. In some other institutions, the 10+2 pass percentage required is 55%. Whereas some colleges consider physics, chemistry and biology combination of subjects as a basic entry level qualification and others take into account the physics, chemistry and mathematics combination. Once this eligibility criterion is met, you can get admission in to BSc programs.

However, for many undergraduate courses in biotechnology you need to sit for common engineering entrance exams such as:
•  The Joint Entrance Examination for IITs (IITJEE): for admission to various IITs
•  All-India Engineering Entrance Exam (AIEEE): for admission to various National Institutes of Technology or regional engineering colleges

Other than these common entrance examinations, engineering institutions offering undergraduate programs conduct their own entrance examinations. The result of these entrances will allow you get admission to an undergraduate course in biotechnology in reputed engineering colleges.

Once you have earned a graduate degree in this field you can go for the master’s level (MSc, ME, MTech). For a Masters in Biotechnology, you should either have a graduate degree in biotechnology or in any subject that is related to biological sciences. A graduate degree in subjects such as biochemistry, biology, botany, chemistry, microbiology, pharmacy, veterinary sciences or zoology is acceptable for an entry into a post-graduate program in biotechnology.

Apart from undergraduate programs, many colleges and universities offer five and five-and-a-half year integrate programs (BTech/MTech) for students. It should be kept in mind that a post-graduate qualification in biotechnology is a must to enter the profitable and ever-expanding field of biotechnical applications.

As it is a blend of numerous disciplines, you have to do a specialisation in a number of fields. Some of the common areas of research include fields like: Agriculture and Agricultural Development, Animal Husbandry, Biochemistry, Biophysics, Botany, Dairy Technology, Environmental Protection, Fishery Development, Genetics, Horticulture, Medicine, Microbiology, Molecular Biology, Nutrition, Pharmacology, Tissue Culture and Zoology.

After post-graduation, you can apply for a doctoral program and from then on if need be, immerse yourself in post-doctoral research.  Here, it is important to point out that a majority of students prefer to do their PhD and post-doctoral research from foreign universities.

Start Early

To follow biotechnology as a preferred profession, you must have a sound grounding in science from the early school days. You must have keen interest in various branches of science not just for study sake or for obtaining good grades. Rather, you must have a keen perception to understand scientific applications and even keep pace with the latest news from the scientific world.

Once you are through with 10+2, you must either opt for an undergraduate course in biotechnology or any subject that is directly or indirectly related to biological sciences. Remember a masters degree is a must to make a successful career in the domain of biotechnology.

Is it the Right Career for Me?

Important traits for getting into this domain are a high degree of intelligence and a general aptitude for science and scientific applications.

Since biotechnology is redefining the boundaries of science, to become a part of such an innovative field you must posses an inherent attitude of originality and imagination. Other skills that will sail you through a successful and a fulfilling career in this path-breaking area are perseverance, immense patience, analytical proficiency, ability to work for long hours, team spirit and communication skills.

What would it Cost Me?

A typical course in biotechnology can annually cost you anywhere between Rs 20,000 to Rs 2,00,000 or above depending on the college you study.

Usually, government and regional colleges that select students through tough entrance exams charge less as compared to programs offered by private owned colleges.

Funding/Scholarship

Various academic institutions and universities offer scholarships to deserving students who perform extraordinarily in the entrance test and post-entrance  interviews. However, easy loans are available from banks for students to pursue their academics. The money borrowed may be returned after a student gets a job.

For instance, State Bank of India, Allahabad Bank, State Bank of Mysore and Bank of Baroda are some popular banks that offer attractive loans to students. These loans include library fees, book purchases, travel expenses and often allow students to purchase computers, accessories and scientific instruments required to complete their work.

Job Prospects

Biotechnology assimilates in itself a number of disciplines. Further, there is a great demand for biotechnical experts in countless industries and sectors. The following are applications where biotechnological techniques are used extensively:
• Agriculture
• Animal Husbandry
• Environment Conservation
• Genetic Engineering
• Health Care
• Medicine
• Industrial Research and Development

Career opportunities for students in biotechnology abound. Those specialising in different sub-disciplines of this field can easily find jobs in both private and government sector undertakings. If you have acquired a post-graduate degree in biotechnology then you can easily find a suitable position in a number of industries.

Major recruiters include industries engaged in processing and developing agricultural and biological products, bio-processing industries, pharmaceutical and chemical industries. There are ample opportunities in industries producing healthcare products, textile industry, cosmetics and organisations engaged in different types of industrial research and development.

These days a growing number of qualified biotechnical professionals are engaged by different industries for environment protection activities and for the safe disposal of hazardous materials.

Pay Packet

Like any other job profile, the pay packet largely depends on your academic qualification, your area of specialisation and the institution from where you have earned your degree. Fresh graduates in this field can generally bag a monthly salary in the range of Rs 8,000 to Rs 20,000.

It has been observed that candidates with a Masters degree in Biotechnology get a better starting salary as compared with students who just have a graduate degree in this field. With an increase in the level of experience, there is abundant scope for getting fatter pay cheques in this exciting and inspiring profession.

Demand and Supply

A number of factors determine the demand of professionals from this interdisciplinary field. India primarily being an agrarian economy, requires professionals who can improve agrarian practices and optimise agricultural output. The growing population of the country requires improved life sciences related products and medicines. This in turn fuels the demand for an increased research and development of vaccines, medicines, tissue culture methods and so on.

There is a steady demand for these professionals in numerous organisations engaged in different types of industrial research and development. On the supply side, technical colleges are trying their best to meet the growing demand for qualified professionals.

One major problem faced on the demand side is that a vast majority of students who are engaged in cutting edge research often tend to leave India to complete their research projects. Moreover, once these students complete their research work they tend to stay and work abroad.

Market Watch

Biotechnology has been making steady progress in last decade and a half. More than half of the earnings from this industry currently come from exports and the trend is expected to remain the same in the coming years.

Although, students doing research work in this field have a tendency to work in foreign countries, yet there is huge potential for students who wish to stay and work in India. According to a recent survey, India is stated to become an international focal point for development of biotechnology.

The areas where biotechnology has grown in India includes Agricultural Biotechnology, Animal Husbandry, Bioinformatics, Bio fertilizers and Dairy development. Growth has also been seen in the area of Bio-resource Development, Plant Biology, Marine Biotechnology, Microbiology, Genetic research.

The changes in the patent regime along with the initiatives taken by the government are expected to bear fruit. All this will lead to a rapid growth and expansion of biotechnological industries in the country.

International Focus

At the international level, India has started making its presence felt in the domain of biotechnology. However, there is tremendous scope for graduate and post-graduate students from this field especially in the United States and in European countries.

A large number of post-graduate students and research scholars tend to move out of India to complete their higher studies. Furthermore, these students prefer to seek employment in the country where they complete their studies or research projects.

Positives/Negatives

+ves:
• This is an upcoming and constantly growing science and has worldwide scope especially in terms of research.

• The scope for research is very wide and efficient work gets fast acclamation within multinationals.

• If you succeed in clearing the tests and interviews, it is assumed you are the right fit for the profession.

-ves:

• The number of seats for biotechnology courses is very limited across colleges in comparison with subjects such as physics, chemistry or biology.

• The testing and interviewing processes usually are difficult and one has to be technically expert.

• The number of companies employing biotechnology students is less when compared with number of companies hiring IT professionals or finance professionals.

• You will have to put in a lot of hard work which can be frustrating at times.

• This is not a profession for people who want to make fast buck but for people who are looking for a long and a well charted career.

Different roles, different names

Biotechnology, as the name suggests is the technology of biology and the scope of both put together. On the one hand, it has concepts from biology explored in depth. On the other hand, this subject explores the impact and the influence of technology on the subject matter. The subject is quite similar to Bioinformatics, which explores biological information in depth using analytical and scientific tools.

Top Companies

Some companies to work with:
1. Biocon
2. Serum Institute of India
3. Panacea Biotech
4. Mahyco Monsanto Biotech
5. Rasi Seeds
6. Novo Nordisk
7. Aventis
8. Indian Immunologicals
9. Venkateshwara Hatcheries
10. Ranbaxy
11. Dr. Reddy’s Labs
12. Piramal Healthcare.
Overall, these are some of the major names in Indian biotechnological industry which extensively use biotechnological processes and techniques to develop and innovate products and processes.

Tips for Getting Hired

1. Apply to at least five to ten top companies to have options for selecting the best job offer out of the ones received.

2. Carry proof of your technical expertise and assure as much as possible that you will be an asset to the company that hires you.

3. Demand a reasonable salary as in the beginning, your focus must be to learn how the industry works, overall.

4. Explore all the possible learning and development opportunities that the companies you have applied to, will provide you. This will help you in choosing the best out of all the offers received.

Protein Folding via Charge Zippers

Membrane proteins are the “molecular machines” in biological cell envelopes. They control diverse processes, such as the transport of molecules across the lipid membrane, signal transduction, and photosynthesis. Their shape, i.e. folding of the molecules, plays a decisive role in the formation of, e.g., pores in the cell membrane. In the Cell magazine, researchers of Karlsruhe Institute of Technology and the University of Cagliari are now reporting a novel charge zipper principle used by proteins to form functional units (DOI: 10.1016/j.cell.2012.12.017)

Like the teeth of a zipper, the charged amino acids (red, blue) form connections between protein segments. In this way, they can form pores in the cell membrane. (Figure: KIT)

“It is fascinating to see the elegant basic principles that are used by nature to construct molecular assemblies,” explains Anne Ulrich, Director of the KIT Institute for Biological Interfaces. “A charge zipper between the charged side chains is an entirely unexpected mechanism used by membrane proteins to neutralize their charges such that they can be immersed into hydrophobic cell membranes.”
In the study published now, Ulrich and her team investigate the so-called Twin-arginine translocase (Tat) that is used in the cell membrane of bacteria as an export machinery for folded proteins. Several TatA subunits assemble as a pore that can adapt its diameter to the size of the cargo to be transported. “But how can such a pore be built up from TatA proteins? How can they reversibly form a huge hole in the membrane for a variety of molecules to pass through, but without causing leakage of the cell?”, Ulrich formulates the questions studied.
To answer these questions, the researchers studied the molecular structure of TatA protein from the bacterium B. subtilis, which consists of a chain of 70 amino acids. The analysis showed that it folds into a rather rigid, rod-shaped helix that is followed by a flexible, extended stretch. Many amino acids in the helix and the adjacent stretch carry positive or negative charges. Surprisingly, the sequence of charges on the helix is complementary to those in the adjacent stretch of the protein. When the protein is folded up at the connection point like a pocket knife, positive and negative charges will always meet and attract each other. Hence, the protein links up both of its segments, similar to the interlocking teeth of a zipper.
“The clou is that this binding principle also works with the neighboring proteins,” Ulrich says. Instead of folding up alone, every TatA protein also forms charge zippers with both of its neighbors. Computer simulations showed that this leads to stable and, at the same time, flexible connections between the adjacent molecules. In this way, any number of proteins can be linked together to form an uncharged ring, which thus lines the TatA pore in the hydrophobic membrane. This novel charge zipper principle does not only seem to play a role in protein transport, but also in the attack of certain antimicrobial peptides on bacteria, or in their formation of biofilms as a response to stress.

source :http://www.kit.edu/visit/pi_2013_12526.php

Study Quantifies the Size of Holes Antibacterials Create in Cell Walls to Kill Bacteria

The rise of antibiotic-resistant bacteria has initiated a quest for alternatives to conventional antibiotics. One potential alternative is PlyC, a potent enzyme that kills the bacteria that causes strep throat and streptococcal toxic shock syndrome. PlyC operates by locking onto the surface of a bacteria cell and chewing a hole in the cell wall large enough for the bacteria’s inner membrane to protrude from the cell, ultimately causing the cell to burst and die.

Research has shown that alternative antimicrobials such as PlyC can effectively kill bacteria. However, fundamental questions remain about how bacteria respond to the holes that these therapeutics make in their cell wall and what size holes bacteria can withstand before breaking apart. Answering those questions could improve the effectiveness of current antibacterial drugs and initiate the development of new ones.

Researchers at the Georgia Institute of Technology and the University of Maryland recently conducted a study to try to answer those questions. The researchers created a biophysical model of the response of a Gram-positive bacterium to the formation of a hole in its cell wall. Then they used experimental measurements to validate the theory, which predicted that a hole in the bacteria cell wall larger than 15 to 24 nanometers in diameter would cause the cell to lyse, or burst. These small holes are approximately one-hundredth the diameter of a typical bacterial cell.

“Our model correctly predicted that the membrane and cell contents of Gram-positive bacteria cells explode out of holes in cell walls that exceed a few dozen nanometers. This critical hole size, validated by experiments, is much larger than the holes Gram-positive bacteria use to transport molecules necessary for their survival, which have been estimated to be less than 7 nanometers in diameter,” said Joshua Weitz, an associate professor in the School of Biology at Georgia Tech. Weitz also holds an adjunct appointment in the School of Physics at Georgia Tech.

The study was published online on Jan. 9, 2013 in the Journal of the Royal Society Interface. The work was supported by the James S. McDonnell Foundation and the Burroughs Wellcome Fund.
Common Gram-positive bacteria that infect humans include Streptococcus, which causes strep throat; Staphylococcus, which causes impetigo; and Clostridium, which causes botulism and tetanus. Gram-negative bacteria include Escherichia, which causes urinary tract infections; Vibrio, which causes cholera; and Neisseria, which causes gonorrhea.

Gram-positive bacteria differ from Gram-negative bacteria in the structure of their cell walls. The cell wall constitutes the outer layer of Gram-positive bacteria, whereas the cell wall lies between the inner and outer membrane of Gram-negative bacteria and is therefore protected from direct exposure to the environment.

Georgia Tech biology graduate student Gabriel Mitchell, Georgia Tech physics professor Kurt Wiesenfeld and Weitz developed a biophysical theory of the response of a Gram-positive bacterium to the formation of a hole in its cell wall. The model detailed the effect of pressure, bending and stretching forces on the changing configuration of the cell membrane due to a hole. The force associated with bending and stretching pulls the membrane inward, while the pressure from the inside of the cell pushes the membrane outward through the hole.

A transmission electron microscope image of a Streptococcus
 pyogenes cell experiencing lysis after exposure to the
highly active enzyme PlyC. (Credit: Daniel Nelson, UMD)

“We found that bending forces act to keep the membrane together and push it back inside, but a sufficiently large hole enables the bending forces to be overpowered by the internal pressure forces and the membrane begins to escape out and the cell contents follow,” said Weitz.
The balance between the bending and pressure forces led to the model prediction that holes 15 to 24 nanometers in diameter or larger would cause a bacteria cell to burst. To test the theory, Daniel Nelson, an assistant professor at the University of Maryland, used transmission electron microscopy images to measure the size of holes created in lysed Streptococcus pyogenes bacteria cells following PlyC exposure.

Nelson found holes in the lysed bacteria cells that ranged in diameter from 22 to 180 nanometers, with a mean diameter of 68 nanometers. These experimental measurements agreed with the researchers’ theoretical prediction of critical hole sizes that cause bacterial cell death.
According to the researchers, their theoretical model is the first to consider the effects of cell wall thickness on lysis.

“Because lysis events occur most often at thinner points in the cell wall, cell wall thickness may play a role in suppressing lysis by serving as a buffer against the formation of large holes,” said Mitchell.
The combination of theory and experiments used in this study provided insights into the effect of defects on a cell’s viability and the mechanisms used by enzymes to disrupt homeostasis and cause bacteria cell death. To further understand the mechanisms behind enzyme-induced lysis, the researchers plan to measure membrane dynamics as a function of hole geometry in the future.
source:http://www.gatech.edu/newsroom/release.html?nid=182231

Team Zone A

Team Under

Asif Raazaq, Co-Founder/Director



Shruti Thakur, Regional Head Delhi NCR
Stuti Mahajan, Regional Chief Editor Delhi NCR
Avantika Rawat, College Head Delhi NCR (JIIT)
Jahnavi Sharma, College Head Delhi NCR (JIIT)
Deepali Gupta, Regional Head- Research and Development Delhi NCR
Pawan Kushwaha, Regional Head-Tech and Operation U.P.
Divyanshi Yadav, Regional Head Research and Development U.P.
Ambuj Mishra, Regional Head External Relations, U.P.
Abhishek Singh, Regional Head Media and Advertising, U.P.
Wasi Syed, Regional Head Research and Development, Punjab
Sanjana Vig, Volunteer
Kalyani Verma, Volunteer


Team Zone N

North Team under

Nimish Gopal, Co-Founder/Director
Tushar Kant, Chief Designer (Former)
Abin Ghosh, Regional Head-Technology and Maintenance

Mohd Tayyab, Regional Head-Editor

Rajat Yadav, Regional Head-External Relations

Naveen Nagar, Regional Head-Research and Development

Harsh Patodia, Regional Head-Finance

Teena Mehlawat, Regional Head-Rajasthan

Tanvi Das, Editor-GBioFin


Anurag Tiwari, Student Head-Haryana
Designer team- Manohar, Arun, Saurabh, Akshay, Moinak


GEIC deadline extended to 31st December


   
                                                                                           


GEIC

    GBioFin Entrepreneurship and Innovation Certificate

    NOW IT’S YOUR TURN TO BE THE NEXT KIRAN MAZUMDAR!!!!!!!!!!!


We come across many students who have lots of business ideas during their graduation days, about opening their own business and a dream of being the Boss /CEO of their own company. But with time, these ideas get cornered and a person ends up finding a good job or takes up higher studies etc, but very few have the courage to start their own venture.

This happens due to many reasons like
a) Lack of knowledge like about how to go into the business
b) Improper direction as to what are the requirements for starting a business 
c) No guidance or less courage to start their own venture.

GBioFin (Gate to Biotech Industries, Organizations and Foundations in India) proudly announces the launching of GEIC (GBioFin Entrepreneurship and Innovation Certificate), a program specialized for entrepreneurship learning.
GEIC is 3 months Online Program started for promoting Biotechnology and Life Sciences in Entrepreneurship field. GEIC aims to provide all the Information regarding Entrepreneurship, Innovation, Research, and Intellectual Property Rights and so on.


It’s a 3-month online course, in which we will give you a larger and a practical picture of the steps / requirements that you need to go through before starting your own venture.
This 3 month program  will give you an edge over other students. Usually, students do  this kind of course after graduating from college so getting this know-how at the college level will at least save 1.5 years of your life.

Programme Includes-
     1. Online Interactive Sessions
     2. Online best course material and module notes (based on patterns from Cambridge and Oxford)
     3. E-Copies of Quarterly Magazine(Biotech Rings) for all the registered  students.
     4. Launch of a student Educational Magazine (E-Copy) for GEIC enrolled Students(including Articles given as assignment for GEIC)
     5.  Interaction with the advisor assigned for the GEIC through email
     6. GEIC Certificate will be sent at the home addresses of each candidate.
     7. Entry in the GBioFin Events with Heavy Discount/ Free Entry in some cases
     8. Gate100 Online Study materials for all.

And many more…

Registration Fees= 1000 /-

To register for GEIC please visit our website www.biofin.net, all the information and details are provided on the homepage.

Contact us:-
Shah Vaibhav-   09769253423
Co-Founder GBioFin Biotechnology Services

TATA First Dot Student Start-up Award

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VOTE for us  ( GBioFin Biotechnology Services) at Tata first dot to BRING THE CHANGE
Get yourself registered  and VOTE for Biotech.
Remember “VOTE” is different from the “LIKE” option. So do cast your vote. AND BRING THE CHANGE !!!!!!!
3 Steps to Cast your VOTE :-
1)Click on the link http://tatafirstdot.com/nomination/gbiofin
2)Click on the Thumbs up Option
3)Sign Up and Like again on the FB Link ( confirmation link will be send to your email id)
4) After the Click on the confirmation link your will be able to CAST YOUR VOTE
5) After you VOTE , make sure your Vote is registered.
– Make sure you VOTE and not just Like 

We have got 4,195 members on Facebook group. We can win this. Please vote.

Come Join Us in our Movement to BRING THE CHANGE !!!!!!!

CSIR UGC NET(Life Sciences)

This post is dedicated to all the NET aspirants who will be taking the test this Sunday i.e. the 23rd December 2012. 
We hope you are all geared up and ready to rock the test.
CSIR NET exam is really a tough one and thousands of aspirants desire to crack it. It needs a thorough preparation which you all must be done with by now and a well planned strategy for taking the exam.
Here in this post we will be providing you some tips about CSIR UGC NET.
1. The first suggestion to all is to keep a check on the CSIR HRDG website always, even after you have taken the exam.
For instance the timings of the exams was changed as given here.
2. Try doing a lot of question papers of the previous NET exams, as many as possible. This helps in improving your speed which is a crucial factor when one is attempting the questions in very limited time.
In addition to that, it helps in preparing a strategy which you are most likely to follow in your exam to manage your time between questions (not all questions need to be attempted) and obtain the maximum efficiency and as a consequence the maximum marks.
3. Work on your strong topics very exhaustively. Since only 15/20, 35/50 and 25/75 questions need to be done, it will help you to decide the questions quickly thus saving a lot of time. In other words you should have complete knowledge of the topic you are very good at.
4. Never answer the questions randomly. Answer only if you are sure of the answer.
Sometimes categorizing the questions into 3 categories, absolutely sure ones, doubtful ones and the ones with no idea; is helpful. This way you can attempt the first category and only if the required number of questions have not been attempted then only you should try the next category questions.

Last of all, GBioFin wishes “All the Best” to all the candidates.
Just for reference sake:
Syllabus change 
Some Books for reference :
1. Upkars CSIR-UGC/NET/JRF/SLET Life Sciences by Dr.A.P.Singh Kumar Pushkara
2. Truemans UGC-CSIR JRF/NET Life Sciences by Pramod Singh